# [

###### Abstract

The use of tank-treading as a means of propulsion for microswimmers in viscous shear flows is taken into exam. We discuss the possibility that a vesicle be able to control the drift in an external shear flow, by varying locally the bending rigidity of its own membrane. By analytical calculation in the quasi-spherical limit, the stationary shape and the orientation of the tank-treading vesicle in the external flow, are determined, working to lowest order in the membrane inhomogeneity. The membrane inhomogeneity acts in the shape evolution equation as an additional force term, that can be used to balance the effect of the hydrodynamic stresses, thus allowing the vesicle to assume shapes and orientations that would otherwise be forbidden. The vesicle shapes and orientations required for migration transverse to the flow, together with the bending rigidity profiles that would lead to such shapes and orientations, are determined. A simple model is presented, in which a vesicle is able to migrate up or down the gradient of a concentration field, by stiffening or softening of its membrane, in response to the variations in the concentration level experienced during tank-treading.

Tank-treading propulsion in viscous flows] Tank-treading as a means of propulsion in viscous shear flows P. Olla]PIEROOLLA

## 1 Introduction

Microorganisms such as bacteria and protozoa are able to swim in a viscosity dominated environment through a variety of strategies. Some of them, such as amoebae and some bacteria, exploit deformations in their main body Berg (1976), others utilize cilia Blake (1971); Blake & Sleigh (1974) or flagella Blum & Hines (1979); Berg (2004), still others, such as cyanobacteria, are able to generate travelling waves on their surface Ehlers et Al. (1996). In all cases, contrary to what happens at macroscopic scales, fluid inertia plays no role, and microscopic swimming is essentially a low Reynolds number affair Lighthill (1957); Childress (1981) (see Lauga & Powers (2009) for a recent review).

One of the motivations for the interest in swimming at low Reynolds numbers is its relevance for the future realization of artificial microswimmers, which would have widespread applications in medicine and in the industry. Over the years, various propulsion schemes have been proposed, both discrete (typically, an assembly of rigid parts hinged together, or connected through immaterial links and springs; see e.g. Purcell (1977); Najafi & Golestanian (2004); Avron et Al. (2005)) and continuous Lighthill (1952); Stone & Samuel (1996); Ishikawa & Pedley (2008). In all cases, proper design of a microswimmer entails a complex optimization problem, which must take into account limitations, such as those imposed by the scallop theorem Purcell (1977); Shapere & Wilczek (1989).

Recently, progress in mechanical manipulation at the microscale has allowed to realize the first examples of artificial microscopic swimmers Dreyfus et Al. (2005); Yu et Al. (2006); Behkam & Sitti (2006); Tierno et Al. (2008); Leoni et Al. (2009). At the present stage, however, most of such artificial swimmers are driven by external fields and the problem of an autonomous power source remains under study. Among the solutions that have been taken into consideration, various methods of rectification of Brownian motion Lobaskin et Al. (2008); Golestanian & Ajdari (2009), and mechanical reactions in the swimmer body, induced by inhomogeneity in the environment, e.g. a chemical gradient Golestanian et Al. (2005); Paxton et Al. (2006); Pooley & Balazs (2007).

Given the fact that a microswimmer typically lives in a non-quiescent environment, a possibility that has been taken into consideration, is to exploit the velocity fields already present in the fluid as an energy source for propulsion. Such a swimmer would sail through the fluid, by a sequence of deformations induced in its body by the hydrodynamic stresses in the external flow. A recent example of such “passive” swimming has been illustrated in Olla (2010), based on a discrete swimmer design similar to the one considered in Najafi & Golestanian (2004) and Golestanian & Adjari (2008).

It should be mentioned that passive swimming (at microscopic scales) already exists in nature. An example is the Fahraeus-Lindqwist effect Vand (1948): a red cell in a small artery will deform in response to the flow, in such a way to be pushed to the vessel center, thus decreasing its fluid-mechanic resistivity. In analogous way, vesicles are able to migrate transverse to a wall bounded shear flow thanks to tank-treading Olla (1997); Sukumaran & Seifert (2001); Abkarian et Al. (2002), and similar behavior have been observed in quadratic shear flows as well Olla (2000); Coupier et Al. (2008); Danker et Al. (2009).

Tank-treading has already been taken into consideration as a possible microswimmer propulsion system Purcell (1977); Lehsansky & Kenneth (2008) (see also Tierno et Al. (2008) for a somehow related approach). It is not too much of a surprise, therefore, that the optimal strategy for a passive discrete microswimmer in a viscous flow turns out to be a discrete version of tank-treading Olla (2010). It is natural to ask what would be an appropriate design for a continuous counterpart of this device. We shall concentrate our analysis on continuous microswimmers whose basic structure is that of a vesicle.

One of the motivations for the present study is that the efficiency of a discrete swimmer, of the kind considered in Najafi & Golestanian (2004) and Golestanian & Adjari (2008), is rather low. It is in fact , where the size of the moving parts, is the stroke amplitude, is the body size; typically: . In the case of a continuous swimmer, instead, and the efficiency would become .

The complicated problem lies in the design of an appropriate control system for such a device. In the absence of a control system, a simple vesicle, immersed in a linear shear flow, will stay naturally in a tank-treading condition, provided the viscosity contrast between interior and exterior fluid is not too high Kraus et Al. (1996). A sketch of a tank-treading vesicle in a linear shear is provided in Fig. 1.

Tank-treading will make such a vesicle migrate away from a solid wall perpendicular to the shear plane and parallel to the flow, but, unfortunately, no other migration behaviors are possible. Migration towards a wall, for example, would require an impossible condition, in which, the tank-treading vesicle maintains an ellipsoidal shape, with long axis aligned with the contracting direction of the strain component of the shear. For similar reasons, no transverse migration would be possible in an unbounded shear flow. The missing ingredient is some mechanism to generate internal stresses that counteract the straining action of the external flow.

We want to explore the possibility that a vesicle be able to generate such stresses through appropriate modification of its membrane properties, namely, local stiffening or softening of its outer surface. (We hypothesize that the energy required for stiffening and softening of the membrane be negligible compared to the one that would expended to achieve an identical migration velocity without the help of the external flow; in this sense, we speak of a passive swimmer. Although natural at the macroscale, this hypothesis may require further justification in the case of microscopic objects). Notice that the presence of a mere inhomogeneity in the membrane, say an inclusion, would be insufficient to this goal. Such an inhomogeneity would be advected by the membrane flow and would be unable to lead to a stationary vesicle configuration. The inhomogeneity of the membrane must not vary with time in a laboratory reference frame, which implies that the membrane elements must continuously change their properties, as they tank-tread around the vesicle.

The mechanism through which local modifications of the membrane stiffness lead to the generation of stresses in the fluid, closely resembles the one responsible for the Marangoni effect Young et Al. (1959); Subramanian & Balasubramaniam (2001). Marangoni effects have been considered indeed as a possible mechanism of self-propulsion for droplets in inhomogeneous environments Kitahata et Al. (2002); Furtado et Al. (2008), and Hanna & Vlahovska (2010) have studied the Marangoni stresses, generated by an external shear induced redistribution of a surfactant over a droplet.

The philosophy in the present paper is different: the membrane stresses do not act directly, through internal flows in the vesicle, to generate propulsion; they rather contribute to modify the vesicle shape, and propulsion is achieved through interaction between a fixed non-spherical shape and the external flow.

We shall consider the case of an ideal membrane, so that the only material property that must be taken into account is a bending rigidity. The specific behavior we shall be interested in is the transverse drift in an unbounded linear shear, already considered in Olla (2010). We shall determine the bending rigidity profile that would generate such behavior, and investigate the possibility that the required bending rigidity profiles be obtained as a direct response of the membrane to the external environment, without the need of an “intelligent”, internal control system.

An analytical treatment of the problem is possible only in the case of quasi-spherical vesicles, and, for this purpose, the analysis in Seifert (1999) and Olla (2000) will be generalized to the case of an inhomogeneous membrane. As discussed in Farutin et Al. (2010), the perturbative problem is singular and care must be taken to scale appropriately the shear strength and the viscosity contrast between inner and outer fluid, in function of the deviation from spherical shape of the vesicle. For the sake of simplicity, contrary to Seifert (1999), all finite temperature effect, will be disregarded in the analysis.

This paper is organized as follows. In Sec. 2 the bending forces exerted on the ambient fluid by a quasi-spherical inextensible membrane, will be calculated, generalizing to the case of an inhomogeneous bending rigidity, the analysis in Zhong-can & Helfrich (1989). In Sec. 3, the shape dynamics of a vesicle with an inhomogeneous membrane, in a viscous shear flow, will be analyzed. In Sec. 4, the possibility of drift of a tank-treading vesicle in an unbounded shear flow will be discussed, and the bending rigidity profiles required for drift will be determined. In Sec. 5, a simple model of vesicle, with a membrane that changes properties in response to the external environment, will be presented, and the migration behavior of the vesicle will be discussed. Section 6 is devoted to conclusions. Additional technical details will be presented in the Appendices.

## 2 The inhomogeneous membrane

The shape of a vesicle can be described in terms of the position of the points on the membrane, in function of a suitable set of curvilinear coordinates . For a quasi-spherical vesicle, it is natural to work in spherical coordinates , such that , and we write

(2.0) |

where is the radius of the sphere with volume equal to that of the vesicle.

We can decompose the scalar field in spherical harmonics: . In analogous way, vector fields, such as e.g. the displacement of a membrane point initially at , will be expanded on the vector spherical harmonics basis:

(2.0) |

so that . The basis in (2) can easily be verified to be orthonormal: .

Assuming that the vesicle has volume , it is possible to express the vesicle area in terms of the components through the formula:

(2.0) |

where Seifert (1999). The excess area in Eq. (2), which parameterizes the deviation from spherical shape, will serve as an expansion parameter for the theory. Notice that the terms in the sum in Eq. (2) are identically zero, which reflects the fact, that the components , to lowest order in , correspond to a rigid displacement of the vesicle.

Following Zhong-can & Helfrich (1989), the bending energy of an inhomogeneous membrane can be expressed as surface integral

(2.0) |

where is the mean curvature of the membrane, which can be written in the form Zhong-can & Helfrich (1989): , with is the unit normal and the Laplace-Beltrami operator on the membrane; is called the spontaneous curvature, and is the bending rigidity. For the sake of simplicity, we shall assume symmetry of the membrane, and set . Following Jenkins (1977), to enforce inextensibility of the membrane, we include a position dependent surface tension in the energy integral

(2.0) |

where plays the role of a Lagrange multiplier coupled to the local area element .

Let us suppose the membrane is able to react to the external environment through local variations of its bending rigidity:

(2.0) |

where, as in Goulian et Al. (1993), is assumed small and will serve, together with , as a basis for a perturbation expansion in Eqs. (2). For the moment, we assume the profile to be assigned, and postpone to Sec. 5 any consideration on the dynamical mechanisms determining its form.

The membrane will act on the fluid with a force density

(2.0) |

which will be the sum of a bending force and a tension force . In the case of a homogeneous membrane, the bending force would be directed along the normal to the membrane. The space dependence of produces a tangential force component. In fact, the variation of bending energy produced by a deformation field can be written in the form

(2.0) |

where is the surface element of the undeformed membrane, and subscripts and identify normal and tangential vector components. The tangential contribution in Eq. (2) accounts for the variation of bending rigidity at position , from tangential displacement of a membrane element from position to .

To lowest order in , is a combination of vector harmonics with . From the relation [see Eq. (2)], the tangential component of Eq. (2) has only components from . As it will soon become clear [see Eq. (3.0) below], this implies that flows on the membrane, induced by inhomogeneity of , are necessarily associated with vesicle deformations.

To explicitly calculate the bending force, we expand the mean curvature and the Jacobian in powers of . The mean curvature of a quasi-spherical membrane was calculated in Zhong-can & Helfrich (1989), and can be rewritten in the form:

(2.0) |

where . In analogous way, we can write for the Jacobian:

(2.0) |

Substituting into Eq. (2), we obtain the expression for the bending energy: . Exploiting Eqs. (2) and (2), using the expression , and expanding on the basis (2), we obtain the following expression for the bending force density, valid to lowest order in and :

(2.0) | |||||

To this order of accuracy, the bending force acts on the fluid at the spherical surface . Taking for , the spectrum produced by a discrete set of inhomogeneities (e.g. inclusions) in the membrane, Eq. (2.0) would lead to the zero temperature expressions for the interaction forces among such inhomogeneities, calculated in Goulian et Al. (1993). Notice that inhomogeneity of the membrane produces a tangential component in the bending force, that, in order for membrane area to be conserved, must be counterbalanced by tension forces.

The tension force is obtained from variation of . We restrict our analysis to a situation in which , corresponding to a weak shear regime in which the hydrodynamic and the bending stresses, that must be balanced by , are of the same order [see Eq. (3.0) below]. Global area changes are quadratic in , while local changes are linear, hence, it is convenient to separate in the tension, global and local contributions: . The variation of the anisotropic part is . This leads to the contribution to the tension force, to lowest order in : .

The isotropic contribution to the tension energy is ; its variation is simply , which, from Eq. (2) leads immediately to the result, expanding in vectors spherical harmonics: Seifert (1999). Expanding also in vector spherical harmonics, and summing to , we obtain

(2.0) | |||||

Setting , we obtain the local tension in the absence of external flow. Substituting into the normal component , it is possible to see that the contribution by inhomogeneity of the membrane, to the total normal force , is (we shall identify contributions by inhomogeneity of the membrane, in general, with superscript “”). As it could be expected from analogous behavior in the case of a homogeneous membrane, the components of , associated with rigid displacement of the vesicle, do not contribute to the sum. Notice however that the components of and do not balance in general, and a vesicle with an inhomogeneous, arbitrarily compressible membrane, could propel itself in a quiescent fluid through a mechanism analogous to the Marangoni effect. A combination of inhomoegeneous bending rigidity and surface tension, rather than just an inhomogeneous surface tension (see e.g. Kitahata et Al. (2002)), would be responsible in this case for propulsion. More precisely, it is that is directly responsible for propulsion (we recall that the induced deformation components , to lowest order in , describe rigid displacements), while can be shown to generate flows inside the vesicle, analogous to the convection-like rolls that are present in a droplet experiencing Marangoni propulsion.

## 3 Deformations in an external shear flow

We want to determine the shape evolution equation for a vesicle with inhomogeneous membrane, immersed in the shear flow

(3.0) |

(, and ). The derivation closely follows the one in Seifert (1999), with additional care, in light of the results in Farutin et Al. (2010), given to the singular behaviors taking place in the limit . Identifying with and the dynamical viscosities of the fluid inside and outside the vesicle, we can introduce dimensionless costants, the capillary number and the viscosity contrast :

(3.0) |

parameterizing the relative importance of hydrodynamic forces to internal membrane stresses, and the ratio between internal and external fluid viscosities.

The vesicle will produce a flow perturbation to be added to on the outside of the vesicle, and a flow field inside the vesicle. The boundary condition at the membrane will thus read , and to this we must add the boundary conditions at and at . Let us indicate with capital letters values of the fluid velocity on the membrane. Expanding in the basis of Eq. (2), the boundary condition on the membrane becomes:

(3.0) |

In creeping flow conditions, the viscous forces by the fluid are balanced by the reaction force exerted by the membrane. To lowest order in , the force balance at the vesicle surface, is evaluated at , and the boundary condition equation (3.0) is enforced at as well. To this order in , from membrane inextensibility, such boundary conditions are the ones imposed on the fluid by a rigid spherical surface:

(3.0) |

where superscripts indicate order in . In the following, although and (or ) are not in general quantities of the same order of magnitude, we shall use superscripts to indicate simultaneously order in and [for instance, what we have calculated in Eq. (2.0) is actually ].

We see that . (Besides, it is possible to see that absence of external torques implies to all order in ).

To lowest order in , the force balance equation will take the form:

(3.0) |

where and indicate components of the adimensionalized hydrodynamic surface force density, associated with fluid flow components vanishing, respectively, at and (notice that is defined as as the force density exerted by a flow inside the membrane, whence the minus sign in front of the contribution by ; see Appendix A). Inspection of Eqs. (2.0,A 0-A 0) shows us that, in order for all the terms in Eq. (3.0) to be of the same order of magnitude, the dimensionless parameters and must satisfy:

(3.0) |

corresponding to a regime of weak shear and strong viscosity contrast.

The velocity field determines the membrane dynamics. In particular, the membrane displacement rate obeys the equation Seifert (1999):

(3.0) |

From Eq. (3.0) [see also Eq. (A 0)], is purely due to the vorticity component of , hence, to lowest order in : , where , that is the vorticity part of the shear flow . The advection term in Eq. (3.0) can then be eliminated working in a reference frame rotating with the vorticity of the flow. We thus have, for the component of needed in :

(3.0) |

and we have introduced a superscript as a reminder that the components are calculated in the rotating reference frame.

It is possible to see that local inextensibility leads to the following relation between velocity components on the membrane Seifert (1999); Olla (2000):

(3.0) |

Working in the rotating reference frame, Eq. (3.0) can then be used, together with Eq. (3.0), to express the components in Eq. (3.0) in function of .

The component of Eq. (3.0) can be used at this point to express the local tension in function of the tangential components of and of the hydrodynamic force. Substituting into the component of Eq. (3.0) and using Eq. (3.0) to express in function of , we remain with a first order differential equation for the deformation component . Using Eqs. (2.0-2.0), and (A 0-A 0) to explicitate the various force contributions, we obtain the equation for the deformation dynamics in the rotating reference frame:

(3.0) |

where ,

(3.0) | |||

and the global tension is determined from the constrain equation (2). That all the terms in Eq. (3.0) contribute to the same order in becomes particularly important at the cross-over line in the . where the tank-treading regime [the stationary solution to Eq. (3.0)] is only marginally stable. As discussed in Farutin et Al. (2010), analysis of the crossover region for generic would require inclusion of all terms in Eq. (3.0). The choice allows us to circumvent such difficulties.

For , these equations correspond to the ones obtained in Seifert (1999) and Olla (2000). They differ only for the expression of the coefficient of the time derivative, that, to the order considered in , due to the ordering in Eq. (3.0), must contain only terms linear in .

In order to return to the laboratory frame, it is sufficient to include in the time derivative in Eq. (3.0) the effect of rotation:

(3.0) |

where unless , in which case:

(3.0) |

Equation (3.0) takes then the form in the laboratory frame:

(3.0) |

where now, from Eq. (A 0):

(3.0) |

From Eqs. (3.0) and (3.0), we see that inhomogeneity of the membrane acts in the dynamics as a forcing, which acts side by side with the effect of the external flow. Choosing components appropriately, a tank-treading vesicle in an external shear flow could be stabilized at orientations otherwise impossible to achieve (e.g. an ellipsoidal shape with long axis along the contracting, rather than the expanding strain direction).

## 4 Drift behaviors

Through tank-treading, a vesicle will be able to maintain a fixed shape and orientation in a stationary external flow. In the absence of inhomogeneities in the membrane, a tank-treading vesicle in the shear flow described by Eq. (3.0), will maintain an ellipsoidal shape with long axis somewhere between the stretching direction of the strain and the flow direction Kraus et Al. (1996). A fixed orientation is the main ingredient allowing migration of a tank-treading vesicle across the velocity lines of the shear flow, and a vesicle, in the condition described above, would migrate away from a solid plane wall perpendicular to the axis.

Non-homogeneity of the membrane provides an additional mechanism to control the shape and orientation of a vesicle in an external flow, and could be used in principle to generate drift behaviors that would otherwise be impossible. We shall focus on the problem of generating a transverse drift in the flow of Eq. (3.0), in the case of an unbounded domain.

In order for such a drift to be present, it is necessary that the velocity perturbation has components , , signaling the presence of a net hydrodynamic force acting on the vesicle (see Appendix A). To obtain such harmonics, we must include in the boundary condition Eq. (3.0) determining , the effect of non-sphericity of the surface . The procedure parallels the one in Olla (2000). To , we can write:

(4.0) |

from which we get the boundary condition .

Passing to vector spherical harmonics, Eq. (4.0) will take the form:

(4.0) |

where . Exploiting Eqs. (A 0-A 0) and (3.0), we can write:

(4.0) |

It is possible to see that the contribution to drift from vanishes identically. In fact, in the rotating reference frame, the components , are related to through Eqs. (3.0) and (3.0). From Eq. (3.0), we find in the laboratory frame:

and we see immediately that for ; .

Returning to Eq. (4.0), we see from Eqs. (4.0) and (A 0) that the only surviving terms in the sum are those for , and , . Expanding , the sum to right hand side of Eq. (4.0) reduces essentially to two terms, involving matrix elements:

Substituting, together with Eq. (4.0), into (4.0) and then into Eqs. (A 0-A 0), we finally obtain:

(4.0) |

A similar coupling between the (or ) harmonics in a shear flow and (or ) harmonics in the internal properties of a droplet immersed in the flow, has been shown in Hanna & Vlahovska (2010) to induce transverse migration of the droplet.

From and , we see that a tank-treading vesicle drifting to positive will need to have a shape, whose section in the shear plane (for ) is an egg with the tip at . The geometrical mechanism for drift along is illustrated in Fig. 2 and parallels what is obtained in the case of the discrete swimmer discussed in Olla (2010). Drift to positive will require, on the other hand a shape whose section in the plane is an ellipse with the long axis at with respect to . A discrete version of a passive swimmer undergoing such a kind of chiral migration has been illustrated in Watari & Larson (2009). In both Olla (2010) and Watari & Larson (2009), the drift was generated in an ensemble of connected spheres rotating in a shear flow, imposing a configuration that was on the average asymmetric in the laboratory reference frame.

We can imagine at this point a hypothetical microswimmer, whose structure is that of a vesicle, with full control of the mechanical properties of its membrane, and ask what modification of would be required to achieve the drift behaviors described in Eq. (4.0).

From Eq. (4.0), we see that the drift is maximized if all the excess area is stored in the deformation components . In order for , we need that the forcing from the strain components of in Eq. (3.0), be canceled by the contribution by membrane inhomogeneity. From Eq. (3.0):

(4.0) |

The components of are obtained imposing in Eq. (3.0) the tank-treading condition , together with for . Using Eqs. (3.0) and (3.0):

(4.0) |

We see from the third in Eq. (4.0) that the component depends on tension , which remains undetermined. A lower bound for the amplitude can be obtained requiring stability of the configuration, i.e. , for which it is sufficient that , i.e. . From Eq. (2), we find therefore:

(4.0) |

and the arbitrariness of reflects the independence of the two expansion parameters of the theory and .

## 5 A vesicle that “swims” in response to concentration gradients

A microswimmer, such as the one described in the previous section, would probably require a sophisicated control system to achieve the bending rigidity modifications described in Eqs. (4.0-4.0). One may ask whether a simpler design is possible, in which the membrane reacts directly to the external environment, without the need of an internal control system.

We are going to describe such a design, in which the vesicle is able to migrate up (or down) a concentration gradient in the shear plane, through softening (or stiffening) of the membrane, in response to a local property of the fluid, such as e.g. the presence of a chemical substance, a temperature inhomogeneity, or a light intensity gradient. A key ingredient will appear to be the presence of a delay in the membrane response to the external environment.

Let us assume that the response of a membrane element in our vesicle, to a concentration field , be described by a linear relaxation equation in the form:

(5.0) |

where and could be in general isotropic operators. Equation (5.0) could be seen as the result, say, of a process of absorption or chemical reaction with the environment (the field may describe e.g. an absorption flux from the bulk, would be an absorption constant and may account for surface diffusion effects). The flow on the tank-treading membrane is accounted for by the advection term . In stationary conditions, the time derivative will drop off Eq. (5.0).

Let us assume the presence of a concentration gradient along , so that, on the membrane: . (We assume that the diffusive current responsible for the gradient in is much larger than its advective counterpart , generated by the flow perturbation due to the vesicle). The effect of the constant part is an isotropic contribution to that could be reabsorbed in a renormalization of . To determine the anisotropic part, we expand Eq. (5.0) perturbatively in :

(5.0) |

and so on to higher orders.

Let us focus on the regime in which the relaxation time scale for the membrane properties is much longer than that of the hydrodynamics, that is . In this regime, a membrane will soften (or stiffen) while cruising at , and start stiffening (or softening) when crossing to . In the velocity field described in Eq. (3.0), therefore, our vesicle will present a softer (stiffer) side to , and we would expect an egg shape with tip pointing at (at ). From Eq. (4.0), this would correspond to drift to positive (negative) . Unfortunately, a linear theory, based only on the first of Eq. (5.0), turns out be insufficient to account for this effect.

Proceeding as before, we expand Eq. (5.0) in spherical harmonics. The lowest order contribution to advection reads ; using Eq. (3.0), the first of Eq. (5.0) becomes:

(5.0) |

where now and are numbers. We see immediately that is a superposition of harmonics, which do not contribute, to lowest order in , to the vesicle dynamics [see Eqs. (3.0,3.0,3.0)]. Thus, to lowest order in , the shape of a vesicle in the shear flow of Eq. (3.0) will be the same as in the case of a homogeneous membrane: an ellipsoid with the long axis between the stretching direction of the flow and the axis.

At this point, two strategies are possible: one is to replace Eq. (5.0) by a nonlinear model equation; the other is to take into account higher order terms in in the vesicle response to . Now, an equation like (5.0) describes the response of the membrane to the small variations of that occur on the scale of the vesicle. The physical meaning of a nonlinear version of such equation remains therefore unclear. On the other hand, the higher order terms in the response to , could provide qualitative information on the behavior of strongly non-spherical vesicles. This suggests us to opt for the second strategy, and to focus on the higher order contributions to the membrane response.

We must consider the secondary deformations induced by , and by those non-linear contributions to the -dependent part of the force exerted by the membrane on the fluid, that were disregarded in Eqs. (2.0) and (2.0). Writing in explicit form:

(5.0) |

with identifying the contribution by , accounting for the nonlinear part of the force.

Notice that the force terms in Eq. (5.0) are . To the same order of accuracy, also terms should be taken into account; one example are the corrections from approximating in the Dirac deltas entering and [see Eqs. (2.0) and (2.0)]. However, from symmetry of the flow, terms that do not involve are superpositions of even harmonics, while drift is produced by harmonics [see Eq. (4.0)]. Such contributions to the force can thus be disregarded.

### 5.1 Higher order contributions to the bending rigidity

Let us consider first the contribution to the membrane force from , i.e. the term in Eq. (5.0). First, however, we have to evaluate the components . From , and using Eq. (3.0) in Eq. (5.0), we find, in the limit :

(5.0) |

In order to determine , we have to solve the second of Eq. (5.0). From Eqs. (3.0,3.0) and (3.0), we have . From Eq. (3.0) and the fact that has only components, will be a superposition of components. Substituting into the second of Eq. (5.0), passing to spherical harmonics, and using Eq. (5.0), we obtain therefore:

(5.0) | |||||

where .

We shall need only the components of . Solution of Eq. (5.0), using Eq. (3.0) and the expressions for the matrix elements provided in Appendix C, gives then the result, for :

(5.0) |

The contribution to the bending force is in the same form as Eq. (2.0):

To this, we must add a tension force contribution , whose effect, as in the derivation of Eq. (3.0), is to cancel the tangential part of . Using Eq. (2.0), we obtain