Helical micropumps near surfaces
Recent experiments proposed to use confined bacteria in order to generate flows near surfaces. We develop a mathematical and a computational model of this fluid transport using a linear superposition of fundamental flow singularities. The rotation of a helical bacterial flagellum induces both a force and a torque on the surrounding fluid, both of which lead to a net flow along the surface. The combined flow is in general directed at an angle to the axis of the flagellar filament. The optimal pumping is thus achieved when bacteria are tilted with respect to the direction in which one wants to move the fluid, in good agreement with experimental results. We further investigate the optimal helical shapes to be used as micropumps near surfaces and show that bacterial flagella are nearly optimal, a result which could be relevant to the expansion of bacterial swarms.
Transporting fluid on small scales is an important but complex problem. Various pumping mechanisms are observed in nature, from fluid being pushed by muscles in the walls of the lymphatic vessels kunert2015mechanobiological (), sodium-potassium pumps in the membranes of cells glynn1993annual () and efficient osmotic pumping in plants jensen2012universality () to cilia-induced flows in major organs including the brain faubel2016cilia (), the respiratory system fahy2010airway () and reproductive tracts spassky2017development ().
Some of these transport mechanisms inspired the design of pumps for microfuidic systems which may be used for biological and chemical sensing, drug delivery, molecular separation, amplification, and sequencing nisar2008mems (). There are two major classes of such pumps: mechanical displacement pumps, which apply forces to fluids via moving boundaries, and electro- and magnetokinetic pumps which provide energy to fluids continuously and as a result generate flow abhari2012comprehensive (). Mechanical or electrokinetic micropumps are used in the majority of lab-on-a-chip devices that require powered fluid flow, but the technical challenges and the requirement of external power associated with these pumping devices have impeded their miniaturisation.
Self-powered micropumps have been designed to address some of these issues zhou2016chemistry (). One of the solutions is to build bio-hybrid cell-based actuators. Recently, there has been a growing interest in using live biological cells to produce devices that work as microscopic gears sokolov2010swimming (), microrotors di2012hydrodynamic (), micropumps kim2008microfluidic (), microswimmers steager2011electrokinetic (), and microwalkers carlsen2014bio (). Bacterial carpets, which are surface arrays of fixed bacteria uchida2010synchronization (); martindale2017autonomously (), can also create linear and rotational flows darnton2004moving () and enhance mixing kim2007use ().
In this work, we focus on bacterial micropumps, with their potential to be used for automation of chemistry and biology squires2005microfluidics (). In the one of the first bacterial micropump experiments, Escherichia coli (E. coli) cells were tethered to microchannel walls by a single flagellar filament and the bodies of the cells would rotate at about 10 rps, thereby pumping fluid from one end of the channel to the other tung2003cellular (); tung2006microscale (). In another experiment, cells were attached to the surface by their bodies with flagella free to rotate kim2008microfluidic (), demonstrating that the bacteria are able to self-organise, generating a collective flow that can pump fluid autonomously through a microfabricated channel. Furthermore, the addition of glucose to the working buffer raises the metabolic activity of the bacterial carpet, resulting in enhanced pumping performance.
In this paper we consider the bacterial pumping system described in a recent experimental paper where E. coli bacteria were confined within micro-fabricated structures in a prescribed geometrical configuration and whose flagellar rotation collectively generated flow that can transport materials along designed trajectories gao2015using (). This study naturally raises the question of (i) how the performance of the resulting pumps depends on the detailed geometrical characteristics of the microscopic cages in which the cells are trapped and (ii) how to optimise them. In order to answer these questions, we use the mathematical techniques of resistive-force theory lighthill1976flagellar () and slender-body theory johnson1980 (); tornberg2004simulating () to model the flow induced by the rotating flagellar filaments of the trapped bacteria. We first quantify how the magnitude and direction of the bacteria-driven pump depends on the configuration of the flagella and then address the geometrical optimisation of the helical shapes used to generate the flow.
Ii Flux induced by helix rotating above a wall
In this section we derive a mathematical model to compute the flux produced by a fixed bacterium whose flagellar filaments are rotating above a no-slip wall. Both resistive-force theory (analytical approach) and slender-body theory (computational approach) are used and we compare our results with experimental measurements of Ref. gao2015using ().
ii.1 Flux due to singularities above a wall
Consider a Cartesian coordinate system with unit vectors , as illustrated in Fig. 1.
A point force (Stokeslet) is located at a height above an infinite no-slip wall with normal in a fluid with a dynamic viscosity . Assuming the flow to have no inertia, which is a reasonable assumption on the small length scales of bacteria gao2015using (), the resulting solution to the incompressible Stokes equations has a velocity field denoted by . It is a classical (and exact) result that this point force will then produce a net flux (i.e. flow rate) blake1974 (); blake1974fundamental () given by
which is in the same direction as the force (see Fig. 1). The calculation for the flux is summarised in Appendix A. Physically, while the flow induced by a point force decays as , a point force near a wall decays faster as and since the area of integration scales as distance squared, a net flow rate is obtained in Eq. (1).
Similarly, a point torque (Rotlet) , placed at the same location will produce exactly the net flux
which points in the direction perpendicular to both the torque and the surface (see the derivation in Appendix B).
ii.2 Flagellar flows as one singularity
Most motile bacteria, including E. coli, are propelled by the rotation of helical flagellar filaments berg2008coli (). Each filament is attached to the cell body via a short flexible hook itself connected to a bacterial rotary motor. The so-called ‘normal’ shape of the flagellar filament is a left-handed helix, typically rotated in the counter-clockwise direction when looked from behind the cell. An E. coli bacterium has approximately 4 flagella on the body turner2000real () which normally bundle in the back of the cell forming a thick effective helix berg2008coli ().
The simplest model for the flow induced by a rotating bundle of flagellar filaments near a surface consists of computing the net force, , and torque, which the bundle applies on the fluid and use the result for from the previous section. In this simple model, we neglect the presence of the cell body on the fluid flow because only a small portion of the flagellar bundle is near the cell body while the majority of the bundle is closer to the no-slip wall. Let us then assume we have a perfect helix parallel to a no-slip wall whose axis is at height with the wall at (see Fig. 2).
In order to relate the values of and to the geometry of the helical bundle, we use resistive-force theory (RFT) which captures the density of hydrodynamic forces acting on slender filaments in Stokes flows lighthill1976flagellar (). We denote by the drag coefficient (i.e. force per unit length) for translation of a portion of the filament bundle locally perpendicular to its tangent and the drag coefficient in the parallel direction and we write their ratio as . We will use the drag coefficients obtained by Lighthill lighthill1976flagellar () as
where we denote the dynamic viscosity of fluid, the bundle radius, the helix radius (i.e. the radius of the cylinder on which its centreline is coiled), its pitch and the pitch angle (i.e. ), as shown in Fig. 2. The axis of the helix axis is assumed to be directed along the direction and we use cylindrical coordinates around the helix axis.
It is a classical result lauga2009hydrodynamics () that the force per unit length acting on the fluid by a counter-clockwise (CCW, i.e. in the positive direction) rotating left-handed helix has components
where denotes the angular velocity of the helix. The total force acting on the fluid and the total torque about the axis of the helix is obtained by integrating the force density along the arc length leading to
where is the total contour length of the helix. Using the result of §II.1, these force and torque induce a net flow rate of
ii.3 Flagellar flows as a superposition of singularities
The approach in the previous section modelled the helix as a single force and torque singularity. Alternatively, we may write the helix as a superposition of flow singularities. The centreline of the left-handed helix may be parametrised as with
where denotes the arclength along the helix centreline and time. As a result the total flow rate has
ii.4 Numerical approach: Slender-body theory
In parallel to the analytical approach we may use the improved, computational model termed slender-body theory johnson1980 (). Under this framework, there is a linear relationship between the rigid body motion defined by the centreline velocity and the hydrodynamic force density on the fluid formally written as tornberg2004simulating ()
where the local, , and the non-local operators, , are respectively given by
where is the Stokeslet singularity defined as
where , is the parametric arc length of the object and the constant . The parameter that defines slenderness is , and being the cross-sectional radius and total arc length of the slender object.
We solve Eq. (18) numerically in order to obtain the force distribution on a helix that undergoes rigid-body rotation. The helix is divided into small straight segments over which the force and velocity are assumed to be constant. This gives rise to a linear system of the form that can be solved for the velocity if the force distribution is known, or inverted to find the force distribution if the velocity is known (which is the case here since the rotation is being prescribed).
In order to account for the presence of wall we place the slender helix in a semi-infinite domain above a rigid boundary. The first term in the non-local operator , Eq. (20), contains the self-interactions of the slender filament. To include the presence of the rigid wall at , we place image singularities for a Stokeslet (blake1974, ) on the opposite side of the wall for each element of the slender object so as to satisfy the no-slip boundary condition on the wall exactly. The operator is then modified as,
where is the image of the Stokeslet,
where is the distance from the wall, , and . The tensor takes the value of 1 when and -1 when and is 0 for every other combination. We can then solve Eq. (18) numerically as above using the modified operator . The obtained force distribution is shown in Fig. 3.
We next integrate the force density on the fluid, , along the arc length of the helix to obtain to the total force and torque along the helix axis and the resulting flow rate,
where are the cylindrical coordinates with the flagellar axis along and is the force density component parallel to the no-slip wall at , i.e. .
ii.5 Comparing analytical model with computations
We may compare the results from the analytical RFT model with the numerical SBT approach by using the set of parameters applicable to E. coli bacteria gao2015using (), namely m, m, m, nm, Hz, m. Force distributions parallel (i.e. along the direction) and perpendicular to the helical axis (i.e. along the direction) are shown in Fig. 3. The main difference between the SBT with and without the hydrodynamic presence of the wall is that adding the wall slightly increases the force component and leads to small end effects. The analytical model (RFT) which is much simpler to implement and does not include the effect of the wall is in good agreement with SBT despite a small but systematic overestimation of the force . Interestingly, the forces parallel to the no-slip wall, i.e. , are not very sensitive to the presence of the wall.
ii.6 Comparison between theory and experiments
Using the parameters for E. coli gao2015using (), we may compute the magnitude of the flow rate, , and its direction, , produced by the rotating flagellar filament. Experimentally, one would like to pump the fluid along a specific direction at the maximum rate, say the negative direction. If we define the angle as the angle between the helix axis and the axis such that the flux vector is along negative direction (Fig. 4) and given the definition of shown in Fig. 2 (top) then one should position the cell at the optimal angle such that .
We use our theory and computations to compute the value of this optimal angle, , and the resulting magnitude of the flow rate, . We obtain results in excellent agreement with each other, namely
In Ref. gao2015using (), Gao et. al. conducted experiments with E. coli bacteria and performed numerical simulations on the fluid flow due to trapped bacteria which were placed at angles with respect to the axis (using our notation). It was found that the among those values, the maximum flow rate was obtained for an angle of . Our theoretical approach agrees with their results. Furthermore we are able to predict that the configuration with is the best one for the use of these cells to pumps fluids.
ii.7 Fluid flow visualisation
In order to gain further understanding of the flow due to the superposition of the two flow singularities above the no-slip wall (Stokeslet and Rotlet) we plot in Fig. 5 the streamlines and contour lines of the velocity magnitude, , on the plane at height m above the wall (with geometrical parameters from Ref. gao2015using ()). Specifically, we plot in Fig. 5(a) the flow due to the point force only, in Fig. 5(b) the flow due to the point torque only and Fig. 5(c) shows the superposition of these two flows. As expected the combination of pushing of the fluid along the helix and rotating the helix, we clearly observe a flow occurring at an angle to the helix orientation.
A comparison between the flow field predicted by the analytical RFT model in the case when we pick and the experimental measurements of Ref. gao2015using () in the same configuration is shown in Fig. 6. The flow field predicted by the theory is qualitatively similar to the experimentally obtained one and in both cases we can clearly see the localised flow occurring mostly along the axis. Note that the experimental flow is about half as strong as the theoretically predicted one which is due to the fluid being stopped by the presence of the boxes which trap bacteria and which are absent in our theory (they essentially provide a vertical no-slip wall at m).
ii.8 Dependence on the distance to the wall
So far we have assumed that the distance between the cells and the surface was a fixed height, . If the helical filament was placed closer or further from the bottom surface, how would this impact the value of the optimal angle ? Our theoretical and computational models (RFT and SBT) allow us to investigate how the flux magnitude, , and the optimal angle, , depend on the value of , with results shown in Fig. 7.
We obtain that the analytical and numerical results are in good agreement. Furthermore, the analytical model predicts the optimal angle using to Eq. (12) is given by
which quantifies the relative strength between the flux generated by the helix torque, , and that due to the force above the wall, . We see that the flow rate is systematically overestimated by the analytical model compared to the simulations though the optimal angle is almost identical for both models. This overestimation using the RFT is due to the overestimation of the total force which becomes more important for larger .
Iii Optimal helical form
While the experiments in Ref. gao2015using () exploited the flagella of E. coli in order to generate flows, the recent development of artificial bacteria flagella zhang2010artificial (); tottori2012magnetic () suggests that helical shapes different from the biological ones could also be used in order to induce pumping. In this section we investigate theoretically if a better (artificial) helical filament could be used to drive the flow.
We pose the optimisation problem in the following manner. We wish to maximise the magnitude of the flow rate, , given a constrain on the rate of energy dissipation in the fluid, (which is equal to the rate of working on the rotating helix against the fluid). Using the analytical RFT approximation, the magnitude of the flow rate is given by
while the rate of viscous dissipation in the fluid due to the rotating but not translating helix using Eq. (8) is
Let us assume that we wish to keep the dissipation constant, i.e. , and maximise the magnitude of . Substituting in terms of into the expression for the flux one gets the flow rate now given by
We see that the flux is inversely proportional to the angular velocity , which means that for a given dissipation, , smaller a angular velocity will lead to a bigger torque , i.e. a larger flow rate. This can be achieved, for example, by having a longer helix and thus a larger value of .
For a given total torque on the helix , what is the shape of the helix which produces the biggest flux ? In order to find the answer, we need to maximise the force for the fixed height . We know that is linearly proportional to the angular speed and the arc length . Fixing the values of and sets the dissipation , and we aim to determine the helix radius, , and its pitch, , so that the flow rate is maximal. This minimisation problem may be formulated using the Lagrangian and the Lagrange multiplier given by
We numerically solve the resulting Euler-Lagrange equations based on the RFT model
in order to determine the extrema for the flux. The solution is shown in Fig. 8(a) as a scatter plot for torques in the range pNm.
The extrema points all lie on the fitted line m which can be rationalised as follows. Ignoring the weak logarithmic dependence of on we can in fact find analytically the optimal values of which maximise the flux for a given torque namely
This analytical solution is shown as a black line in Fig. 8(a) and is very close to the blue line (numerical calculations). In this figure we also use the red triangle to denote the radius and pitch for the flagellar filament of E. coli while the green square denotes the optimal radius and the pitch for pumping such that the torque is the same as that generated the E. coli bacterium. In the case of E. coli, it was reported that the torque applied by the rotary motor remains approximately constant up to a relatively high rotation frequency ( Hz at C) chen2000torque (); nord2017speed (), and we use this biological value of 830 pNm for . We may then calculate the radius, pitch, flux and the optimal angle in terms of the fixed quantities for the optimal shape, i.e. when and obtain
Surprisingly, the optimal pitch is independent of the drag ratio but is only a function of the applied torque , the helix length , the rotation frequency and the drag coeficient .
Instead of the analytical modelling approach, we may instead compute the flux as a function of the parameters of the helix, , using the numerical model (SBT) with fixed helix height above the wall m. These results are shown in Fig. 8(b). The contours of the torque as the function of the helix radius and the pitch match well the ones calculated using the analytical model (RFT). In both cases, the contours are almost horizontal when the radius is small, e.g. about m in the case of E. coli. This can be justified by examining the form of torque,
The torque depends weekly on the pitch , especially when the helix radius is small. This means that the contours of are approximately , i.e. horizontal contour lines. The optimal value of the flux, , is plotted in Fig. 8(c) as a function of the applied torque, . We see that the analytical model systematically overestimates the flux compared with the computational approach. Remarkably, the difference between the flow rate produced by the E. coli bacterium and the optimal one for the same applied torque is only , revealing that the bacterial flagellar filaments are almost at the optimal shape to pump the fluid over the surface. How does this result depend on the distance to the surface? We may compute the ratio between the flux due to the optimal artificial helix (force , torque ) versus the flux due to the E. coli shaped helix (force , torque ).
The applied torque calculated analytically is pNm. The biological force is pN while the optimal force is pN. We see in Fig. 8(d) that the percentage increase in flow rate between the biological value and the optimal value in always in the range and increases with the height above the wall. Bacterial filaments thus seem to be nearly optimal for pumping fluid very close to a surface, a result which could be important in the context of efficient expansion of bacterial swarms wu2011microbubbles ().
Our simple analytical (RFT) and computational (SBT) models are able to capture the leading-order physics of the fluid flux produced by a rotating helical bundle of flagellar filaments near a no-slip wall. Fluid is being pumped along the flagellar axis because of the net force acting on the fluid and in the direction perpendicular to the flagellar axis because of the rotation in the presence of the wall. The force produces a flow rate which depends on the distance to the wall , whereas the torque produces the flux ). The combined effect of force and torque implies that the net flow rate is directed at an angle to the flagellum, whose value depends on the helix parameters and its distance to the wall. While our model is limited to the cases where the boundary affecting the fluid flow is a flat no-slip wall, more advanced numerical techniques would be needed to tackle complex boundaries such as channels, corners, irregular surfaces. Importantly, our theoretical predictions agree with the numerical and experimental results in Ref. gao2015using (), and thus provide fundamental understanding of the relationship between orientation and flow pumping, setting the stage for the future development of efficient micropumps not only using bacteria but also other actuation methods (e.g. magnetic).
We next investigate the optimal shape of the helix in terms of its radius and pitch assuming a fixed applied torque , total helical length , angular speed , height above the wall and filament radius . We find that nearly-optimal pumping is achieved when the helix pitch angle is fixed and equal to , where is the ratio between the drag coefficients. With a drag ratio one finds that is close to . While the pitch angle for normal flagellar filaments is close to and is optimised for propulsion spagnolie2011comparative (), the theoretically-optimal pumping helix performs only to better than E. coli depending on the height above the wall. This is a very small difference indicating that bacterial flagella are very efficient micropumps, a result which could be relevant to the expansion of bacterial swarms kearns2010field () or the future development of biological micropumps to be used for automation in chemistry and biology squires2005microfluidics ().
This work was funded in part by an ERC Consolidator grant from the European Union (EL).
Appendix A Point force above an infinite wall at
We are interested in the Stokeslet parallel to the wall, therefore without loss of generality we choose . Let us calculate the flux in the plane perpendicular to the Stokeslet
Expand the flow to the leading order in and integrate to get
Therefore, there is a finite flow rate produced in the direction of the Stokeslet given by
where . Note that the flux is zero if the force is perpendicular to the wall, as expected by symmetry.
Appendix B Point torque above an infinite wall at
Take then the flux produced by this Rotlet is
Expanding the flow to the order in and integrate we obtain
So in general the flux given by
where and here again the flux is zero if the torque is perpendicular to the wall.
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